mouse anti-ep fitc (Burlington Industries)
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Mouse Anti Ep Fitc, supplied by Burlington Industries, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-ep+fitc/mouse+anti+ep+fitc/bio_rxiv__2023__11__15__567218-144-87-96
Average 90 stars, based on 1 article reviews
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Centrifugation:Article Title: SUMO chains depolymerization induces slender to stumpy differentiation in T. brucei bloodstream parasites Article Snippet: BF cells were collected by centrifugation (1000 x g for 10 min), washed with TDB supplemented with glucose 20 mM and fixed with 4% paraformaldehyde (PFA) in PBS for 1 h. Parasites were allowed to bind to poly-L-lysine coated glass coverslips for 30 min and then incubated with 25 mM NH4Cl for 15 min. Permeabilization and blocking were performed with 3% bovine serum albumin (BSA), 0.5% saponin and 5% normal goat serum in PBS for 1 h. Mouse anti- Tb SUMO (1:500) [ ], mouse anti-VSG221 (1:200), rabbit anti-VSG221 (1:500), mouse anti-EP FITC (1:500) (Cederlane Laboratories, Burlington, Canada) or anti-PAD1 [ ] were used as primary antibodies. Incubation:Article Title: SUMO chains depolymerization induces slender to stumpy differentiation in T. brucei bloodstream parasites Article Snippet: BF cells were collected by centrifugation (1000 x g for 10 min), washed with TDB supplemented with glucose 20 mM and fixed with 4% paraformaldehyde (PFA) in PBS for 1 h. Parasites were allowed to bind to poly-L-lysine coated glass coverslips for 30 min and then incubated with 25 mM NH4Cl for 15 min. Permeabilization and blocking were performed with 3% bovine serum albumin (BSA), 0.5% saponin and 5% normal goat serum in PBS for 1 h. Mouse anti- Tb SUMO (1:500) [ ], mouse anti-VSG221 (1:200), rabbit anti-VSG221 (1:500), mouse anti-EP FITC (1:500) (Cederlane Laboratories, Burlington, Canada) or anti-PAD1 [ ] were used as primary antibodies. Blocking Assay:Article Title: SUMO chains depolymerization induces slender to stumpy differentiation in T. brucei bloodstream parasites Article Snippet: BF cells were collected by centrifugation (1000 x g for 10 min), washed with TDB supplemented with glucose 20 mM and fixed with 4% paraformaldehyde (PFA) in PBS for 1 h. Parasites were allowed to bind to poly-L-lysine coated glass coverslips for 30 min and then incubated with 25 mM NH4Cl for 15 min. Permeabilization and blocking were performed with 3% bovine serum albumin (BSA), 0.5% saponin and 5% normal goat serum in PBS for 1 h. Mouse anti- Tb SUMO (1:500) [ ], mouse anti-VSG221 (1:200), rabbit anti-VSG221 (1:500), mouse anti-EP FITC (1:500) (Cederlane Laboratories, Burlington, Canada) or anti-PAD1 [ ] were used as primary antibodies. |

